EXPERIMENTAL GASTROENTEROLOGY

7.Title page: Promising methods for the treatment of dyslipidemia in patients with NAFLD
Burovenko I. Yu. ^1,2, Borshchev Yu. Yu. ^1,2,3,4, Minasian S. M. ^1,5, Protsak E. S. ^1,5, Sheptitskii V. A. ^2,3,4, Galagudza M. M. ^1,5 📧 .
1. Institute of Experimental Medicine, Almazov National Medical Research Centre, 197341, Saint Petersburg, Russian Federation
2. Shevchenko Transnistria State University, 3300, Tiraspol, Moldova
3. Institute of Physiology and Sanocreatology, MD-2028, Chisinau, Moldova
4. Scientifi c Research Center “Probiocode SP”, 143026, Moscow, Russian Federation
5. First Pavlov State Medical University of St. Petersburg, 197022, St. Petersburg, Russia
Keywords: antibiotics, isolated intestinal loop, absorption, monosaccharides, isolated heart, infarct size

Abstract: Aim: we aimed to study the effect of the mixture of three broad-spectrum antimicrobials (amoxicillin, metronidazole, clarithromycin, AMC) on monosaccharide absorption in the isolated intestinal loop and on myocardial tolerance to ischemia-reperfusion injury (IRI). Materials and Methods: experiments were performed on adult male Wistar rats housed in conventional area. Glucose and fructose absorption was studied in the model of in situ isolated intestinal loop according to Thiry-Vella after administration of AMC into the intestinal lumen. Isolated heart studies were done using modified Landendorff setup after oral treatment of the animals with AMC. Results: AMC significantly inhibited glucose absorption in the isolated intestinal loop, mainly due to pronounced reduction of active, sodium-dependent glucose cotransporter 1-mediated transport. AMC also caused inhibition of fructose absorption. The animals treated with AMC showed decreased systolic pressure in the left ventricle up to 20% upon reperfusion, decreased pressure in the left ventricle during global ischemia (by 33-500%), increased coronary flow by 28-82%, and also insignificant trend to greater infarct size. Conclusion: AMC-induced inhibition of glucose and fructose absorption provides evidence for the existence of rapid changes in nutrient absorption, mainly due to inhibition of active transport. AMC treatment also resulted in deterioration of morphological and functional parameters of the isolated heart. The data obtained warrant further studies on the antibiotic-induced associated changes in enterocytes and cardiomyocytes.
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Full text is published :
Burovenko I. Yu., Borshchev Yu. Yu., Minasian S. M., Protsak E. S., Sheptitskii V. A., Galagudza M. M. The study of absorption of monosaccharides in the isolated intestine and resistance of the myocardium to ischemia-reperfusion in rats after administration of antimicrobial agents. Experimental and Clinical Gastroenterology. 2019;163(3): 43–50. (In Russ.) DOI: 10.31146/1682-8658-ecg-163-3-43-50
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8.Title page: The evolution of ideas about medicinal liver damage
Zhukhovitsky V. G. ^1,3 📧 . Kazakova M. V. ¹, Sysolyatina E. V. ^1,4 Loleit R. A.², Lavrikova A. Ya.¹, Sofronov A. V. ², Ermolaeva S. A. ^1,4,
1. “Gamaleya National Research Centre for Microbiology and Immunology”, Ministry of Public Health of Russian Federation, Moscow, Russia
2. “Kinetika Co., Ltd”, Moscow, Russia
3. “Sechenov The First Moscow Medical University”, Ministry of Public Health of Russian Federation, Moscow, Russia
4. “Moscow Institute of Physics and Technology (State Research University)”, Moscow, Russia
Keywords: Helicobacter pylori, cold plasma, bactericidal effect

Abstract: Medicinal liver damage (LPP) is one of the main undesirable effects of pharmacotherapy. The emergence of new drugs on the pharmaceutical market, an increase in the number of LPP caused by herbal preparations and biologically active additives, as well as the expansion of the market for immunobiological drugs, make drugs the most important etiological factors for liver damage. For timely diagnosis of LPP, it is necessary to take into account the totality of risk factors, among which, in recent years, significant attention has been paid to biomarkers. The choice of tactics for the management of a patient with LPP is determined by the nature and severity of the disease, the characteristics of the use of a potentially hepatotoxic drug and the individual characteristics of the patient. The appointment of drugs with high hepatotoxic potential sometimes requires a preventive prescription of pathogenetic agents. When using drugs with low hepatotoxic potential, individual monitoring of biochemical parameters is recommended.
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Full text is published :
Zhukhovitsky V. G., Kazakova M. V., Sysolyatina E. V., Loleit R. A., Lavrikova A. Ya., Sofronov A. V., Ermolaeva S. A. Bactericidal eff ect of low-temperature plasma against Helicobacter pylori in vitro. Experimental and Clinical Gastroenterology. 2019;163(3): 51–57. (In Russ.) DOI: 10.31146/1682-8658-ecg-163-3-51-57
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9.Title page: Protective effect of the new compound - salicyl-carnosine - on the formation of chronic acetate gastric ulcer in rats
Trubitsyna I. E. ¹ 📧 . Stvolinsky S. L. ², Kulikova O. I. ² Fedorova T. N. ², Tarasova T. B. ³, Efremov L. I. ¹, Mikhailova S. F. ¹, Varvanina G. G. ¹,
1. Moscow Clinical Scientifi c practical Center named after A. S. Loginov, Department of health, city of Moscow, 111123, Moscow, Russia
2. “Research Center of Neurology”, Moscow, Russia
3. FGBOU VPO “Mordovian state University. N. P. Ogarev”, Saransk, Republic of Mordovia, Russia
Keywords: antiplatelet agents, aspirin, gastrointestinal ulcer, antioxidants, carnosine, rats

Abstract: Antiplatelet drugs are widely used for the prevention and treatment of vascular diseases of the heart and brain. The most popular drugs are non-steroidal anti-inflammatory drugs, the first place among which still belongs to acetylsalicylic acid (aspirin) and its derivatives. However, along with high clinical efficacy according to target parameters, these drugs have side effects that lead to damage to the mucous membrane of the gastrointestinal tract (GI). In order to eliminate the effects of damage to the gastrointestinal tract with the use of antiplatelet drugs, based on salicylic acid, using a natural dipeptide carnosine, a new compound was synthesized - salicyl-carnosine, which has an antiplatelet effect. A comparative study on carnosine, acetylsalicylic acid and salicyl-carnosine for the healing of ulcerative defect was carried out on the model of acetate gastric ulcer by Okabe. It was shown that salicyl-carnosine was more effective than carnosine both at the stage of ulcer formation (1-5 s) and at the stage of healing (5-10 s). Acetylsalicylic acid in the used model of gastric ulcer not only did not prevent the development of gastric ulcer, but also complicated the healing processes. The results indicate the possible prospects for the use of salicyl-carnosine as an antiplatelet drug in vascular pathologies (cardiac, Central nervous system, etc.), to avoid side effects associated with damage to the gastrointestinal tract, inherent in aspirin and other NSAIDs. Non-steroidal anti-inflammatory drugs.
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Full text is published :
Trubitsyna I. E., Stvolinsky S. L., Kulikova O. I., Fedorova T. N., Tarasova T. B., Efremov L. I., Mikhailova S. F., Varvanina G. G. Protective effect of the new compound — salicyl-carnosine — on the formation of chronic acetate gastric ulcer in rats. Experimental and Clinical Gastroenterology. 2019;163(3):58–64. (In Russ.) DOI: 10.31146/1682-8658-ecg-163-3-58-64
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10.Title page: Disorders of oxidative phosphorylationin the model of chronic enteral insufficiencyand their correction
Kamilova A. T. ¹, Dustmuhamedova D. H. ¹ 📧 . Levickaya Yu. V. ²,
1. Republican Specialized Scientifi c and Practical Medical of Pediatrics, 100174 Tashkent, Uzbetkistan
2. Center of Advanced Technologies under the Ministry of Innovation Development of the Republic of Uzbekistan, 100174 Tashkent, Uzbetkistan
Keywords: Chronic enteral insuffi ency, oxidative phosphorylation, mytochondria, phytoecdysteroid, ecdysten

Abstract: Aim.To study the state of oxidative phosphorylation in a model of chronic enteral insufficiency in the growing rats and to assess the effectiveness of ecdystene in their correction. Materials and methods. Laboratory white mongrel rats were used in the experiments. The animals were divided into 3 groups. 1 group - healthy animals (control). Group 2 - animals on a diet poor in protein (chronic malnutrition), group 3 - animals that received as a corrective drug ecdysthene. Mitochondria were isolated from the liver of 40-day-old rats by differential centrifugation. The parameters of oxidative phosphorylation, respiration rate of isolated mitochondria in the metabolic state V4 and V3, as well as the values of respiratory control and ADP / O were determined. Results. Treatment of rats with protein deficiency for 10 days with the drug ecdystene resulted in a significant increase in the body weight of the rats - an average of 45.5% - and by the 10th day of treatment this value was 71% of the values in the control group (P <0.01). In the group of animals that were on a diet poor in proteins, the oxidative phosphorylation parameters were significantly reduced. The rate of oxygen consumption by the mitochondrial suspension in the functional state of V3 and V4 in sick animals was almost half that in control animals. Respiratory control, in the group of sick animals was reduced by 20%. The ratio of ADP / O was also reduced and was 2.5 vs 2.8 in the control group. When using a corrective ecdystene preparation as a quality, the V3 state was 93.72, V4 was 15.62, and Vdnf was 109.34 ng at O / min / mg protein (P <0.001). The value of the respiratory control was also close to the control and was 6, and the ratio of ADP / O even slightly exceeded the control value and was 3.03 (P <0.05). Сonclusion. We detected a pronounced disconnection of the respiratory chain in the mitochondrial suspension when the animals were kept on a diet poor in proteins and also had a bright corrective effect in the ecdystene. The therapeutic effect of ecdysten revealed its pronounced ability to correct manifestations of chronic enteric insufficiency in growing rats.
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Kamilova А. Т., Dustmuhamedova D. H., Levickaya Yu. V. Disorders of oxidative phosphorylationin the model of chronic enteral insufficiency and their correction. Experimental and Clinical Gastroenterology. 2019;163(3): 65–69. (In Russ.) DOI: 10.31146/1682-8658-ecg-163-3-65-69
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11.Title page: Reducing the size of the experimental liver brush using radiofrequency ablation
Cherkasov M. F. ¹, Aboyan I. A. ², Aboyan M. E. ², Roshak B. V. ^1,2 📧 . Lemeshko S. I. ², Malikov L. L. ², Khanamirova L. Z. ², Buharbaev R. M. ², Melikova S. G. ¹
1. Federal State Budgetary Educational Institution of Higher Education “Rostov State Medical University” of the Ministry of Healthcare of the Russian Federation, 344022, Rostov-on-Don, Russia
2. Сlinical diagnostic center “Zdorovie”, 344011, Rostov-on-Don, Russia
Keywords: Chronic enteral insuffi ency, oxidative phosphorylation, mytochondria, phytoecdysteroid, ecdysten

Abstract: Radiofrequency ablation can achieve a significant reduction of simple liver cysts. The aim of the study was to investigate the effects of RFA in various regimes on the wall of the liver cyst and adjacent tissue, and to define optimal exposure parameters. Materials and methods. An experimental study was performed by constructing of imitational models of a simple liver cyst (group № 1), a biliary cyst (group № 2), and a cyst with high electrical conductivity content (10% NaCl solution) -group № 3. The models were exposed to RFA in the range of temperatures from 50 to 92 °C with duration from 1 to 5 minutes. Then, the postablation changes of the preparations were assessed using histological examination. Results. In all groups, when heated, there was a lag of the temperature of the cystic medium compared with the temperature of the electrode. For models of group № 1, № 2, and № 3, the difference was 19.6 °C, 8.75 °C, and 3.8 °C, respectively. Coagulation necrosis was found in all preparations and was most pronounced in the surface layers of the tissue contacted with the heated medium. The absence of destruction of the adjacent liver tissue in group № 1 was found for RFA in regimes using 61-63 °C for 2 minutes; in group 2 - using 65-75 °C for 3 minutes; in group 3 - using 85 °C (for 2 minutes) or 53-55 °C (for 5 minutes). Conclusion. The destruction of all epithelial cells is achievable with RFA; however, the thermal range between total ablation and the thermal injury of adjacent healthy tissue is narrow. The introduction of 10% NaCl solution can increase the area of necrosis and makes possibility to control the temperature of the cyst. Achieving mild ablation with shallow penetration of necrosis is influenced by the volume and electrical conductivity of the cystic content, adequate control of the temperature in the cystic cavity, and dosed use of RFA power and exposure time.
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Cherkasov M. F., Aboyan I. A., Aboyan M. E., Roshak B. V., Lemeshko S. I., Malikov L. L., Khanamirova L. Z., Buharbaev R. M., Melikova S. G. Reducing the size of the experimental liver brush using radiofrequency ablation. Experimental and Clinical Gastroenterology. 2019;163(3): 70–76. (In Russ.) DOI: 10.31146/1682-8658-ecg-163-3-70-76
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12.Title page: Effect of toxic liver damage on structural changes in mitochondria and intracellular organelles
Lepekhova S. A. ^1,2 📧 ., Goldberg O. A. ¹, Prokop’ev M. V. ², Kurgansky I. S. ², Kireeva V. V. ², Inozemcev P. O. ², Apartsin K. A. ²,
1. Irkutsk Scientifi c Center of the Siberian Branch of the Russian Academy of Sciences (664033, Irkutsk, Russia)
2. Irkutsk Scientifi c Center for Surgery and Traumatology (664003, Irkutsk, Russia)
3. Irkutsk Regional Clinical Hospital (664049, Irkutsk, Russia)
Keywords: ---

Abstract: ------.
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Full text is published :
Lepekhova S. A., Goldberg O. A., Prokop’ev M. V., Kurgansky I. S., Kireeva V. V., Inozemcev P. O., Apartsin K. A. Effect of toxic liver damage on structural changes in mitochondria and intracellular organelles. Experimental and Clinical Gastroenterology. 2019;163(3): 77–80. (In Russ.) DOI: 10.31146/1682- 8658-ecg-163-3-77-80
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