1. GBU the MСRC named after A. S. Loginov DZ Moscow, 111123 Moscow, Russia
2. Peoples ' friendship University of Russia, Moscow, Russia
3. National research Mordovian state University. N. P. Ogaryov, Saransk, Russia

Keywords: mucous membrane of the gastrointestinal tract, autoimmune component, immune system of the gastrointestinal tract, M-cells

Abstract:The mucous membrane of the gastrointestinal tract is an extensive information and communication system that quickly reacts to both external and internal factors of the body, ensuring the maintenance of homeostasis of tissue and blood. The aim of the study was to determine the factors that ensure the appearance of an autoimmune component in damage to the pancreas in rats. Material: manipulations were performed on white Wistar rats weighing 190,0–230,0.Immunization of animals was carried out according to the standard scheme. There were 4 intraperitoneal injections of 2 ml of pancreatic tissue homogenate. Total antibodies (at) to parietal cells (PC) (Parietal Cell antibodies, IgG, IgA, IgM) were determined in plasma by enzyme immunoassay. The animals were divided into one control group and fi ve experimental groups (6 groups of 5 rats in total). Experimental groups: 1st-immunization of intact animals (IM) according to the above scheme. 2nd-acute pancreatitis (AP); 3rd group AP+ IM; 4th-chronic pancreatitis (CP); 5th group-CP+IM. With AP and CP, there is an autoimmune component that interferes with the restoration of damaged pancreatic tissue. Experimental studies have shown that the presence of acute infl ammation, accompanied by full blood vessels, hyperemia, edema, hypoxia, necrosis, death of a large number of cells, the release of cellular elements that are endogenous antigens. Infl ammation of the pancreas stimulates the immune system of the gastrointestinal tract. In addition, factors contributing to the development of an autoimmune reaction are: alcohol, infection: bacterial, viral and/or parasitic.

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Full text is published :
Trubitsyna I. E., Ruchkin I. N., Mikhailovа S. F., Efremov L. I. et al. MALT-the mucous membrane system of the gastrointestinal tract. Experimental and Clinical Gastroenterology. 2018;159(11): 83–87. (In Russ.) DOI: 10.31146/1682-8658-ecg-159-11-83-87
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1. Pirogov Russian National Research Medical University (RNRMU), Moscow, Russia
2. Peoples Friendship University of Russia (RUDN), Moscow 117198, Russia

Keywords: stomach, colon, motor activity, serotonin

Abstract:Trial objective: study the mechanism of multidirectional eff ect of Serotonin adipinate on gastric and colon motor activity. Materials and methods: experiments were carried out on Wistar rats (N83) in surgical anesthesia. An electromyogram and hydrostatic pressure in the cavities of organs was registered using an amplifi er BioAmp ML132 (Adinstruments, Australia), an analog-to-digital converter Maclab 8e (Adinstruments, Australia), a computer Масintosh Performa 6400/180 and a software program Chart 4.2.3. Trial fi ndings: it was established that intra-arterial injection of Serotonin adipinate at a dose of 0.05 mg/kg and 0.1 mg/ kg causes stimulatory and inhibitory gastric reactions; at a dose of 0.15 mg/kg — only stimulatory ones. Colon appeared to have only stimulatory reactions. It was also found that intravenous injection of Serotonin adipinate does not intensify stomach and colon contraction. Conclusion. Organs’ stimulatory reactions occur due to activation of muscle 5HT-receptors, inhibitory ones — due to activation of presynaptic 5HT-receptors, locating on organ’s adrenergic terminals, and ejection of inhibitory transmitter noradrenaline by them. When using Serotonin adipinate in experiment and in clinical practice, it is necessary to take into account that the organs’ eff ect from serotonin administration into body may be dual: inhibitory at low doses and stimulatory at higher ones.

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Full text is published :
Smirnov V. M., Sveshnikov D. S., Kuchuk A. V., Trubetskaya L. V., Mongush M. I., Ignatova E. D. The mechanism of multidirectional eff ects of serotonin on the motility of the stomach and colon. Experimental and Clinical Gastroenterology. 2018;159(11): 88–92. (In Russ.) DOI: 10.31146/1682-8658-ecg159-11-88-92
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