1. Federal State Scientific Institution Irkutsk Scientific Center of Surgery and Traumatology (Irkutsk, Russian Federation)

Keywords:ulcerative colitis,descending colon of rats,technology of the surgery

Abstract:Background: Ulcerative colitis (UC) is a chronic immune inflammatory condition of the colon with an unknown aetiology, leading to disability and reduced quality of life of patients. UC primarily affects young adults. In most cases, inflammatory bowel disease (IBD) debuts at reproductive age. The incidence of UC and severe clinical course has increased overall across the world. The study of the mechanisms of pathogenesis and aetiology of this disease contributes to the development of new effective methods of treatment. Objective: The aim of our study was to develop technology of the surgery directed to induction of reversible ischemic damage, the erosive-ulceration of gut mucosae (descending colon) at rats of the WISTAR line. Materials and methods: Experimental research was done using male rats «Wistar» (n=40), their age was more than 6 months, weight of body is 280 - 350 grams. The animals were separated into 3 groups: 1 - (n=14) Sholimov’s operative method, 2 group (n=16) - model using special technology, 3 group (n=10) - Coopr’s H. S. induction ulcerative colitis. Observation was been done during 10 days. We offered special surgical technique: parietal ligation on every vasa recta of colon descendens of rats the length 3 cm along colon, on the apex vesicae and then vasa recta are cut. Then during 7 days 1% solution of dextran sulfate sodium (DSN) was given for experimental animals after postoperative period. By the histological biopsy of colon mucosa the results were estimated. Results/Conclusion: we have created erosive-ulceration of gut mucosae (descending colon) of rat with productive inflammation, vasculitis and plasmatic impregnation of the vessel walls and intimal thickening. Our model of ulcerative colitis can be used for the development and testing of new methods for the study and treatment of this pathology.

• References total 8 ⇓ :: .
  1. Second European evidence-based Consensus on the diagnosis and management of ulcerative colitis: Definitions and diagnosis, A. Dignass et all // Journal of Crohn’s and Colitis. - 2012. - V. 6 (10). - December 2012. - P. 965 - 990
  2. William A Rose, Kaori Sakamoto, Cynthia A Leifer: Multifunctional role of dextran sulfate sodium for in vivo modeling of intestinal diseases, BMC Immunology 2012, 13:41 doi: 10.1186/1471-2172-13-41
  3. Martina Perse and Anton Cerar: Dextran Sodium Sulphate Colitis Mouse Model: Traps and Trics. J. of Biomedical and Biotechnology, volume 2012, article ID 718617, 13 pages.
  4. Лепехова С. А. Программа стандартных операционных процедур: лабораторные животные (прием, содержание, уход и контроль здоровья животных в вивариях медицинского учреждения): учеб. пособие. Иркутск: НЦРВХ СО РАМН; ИГМУ, 2012; 96 с.].
  5. Шалимов С. А., Радзиховский А. П., Кейсевич Л. В. Руководство по экспериментальной хирургии. - М., 1989. - с. 207
  6. Puneet Kaur Randhawa, Kavinder Singh, Nirmal Singh, and Amteshwar Singh Jaggi. A Review on Chemical-Induced Inflammatory Bowel Disease Models in Rodents. Korean J. Physiol. Pharmacol. Vol. 18: 279-288, August, 2014
  7. W. Elsheikh, K. L. Flannigan, W. Mcknight, J. G. P. Ferraz, J. L. Wallace. Dextran sulfate sodium induces pan-gastroenteritis in rodents: implicationsfor studies of colitis. Journal of physiology and pharmacology 2012, 63, 5, 463 - 469
  8. H. S. Cooper, S. N. Murthy, R. S. Shah, and D. J. Sedergran, «Clinicopathologic study of dextran sulfate sodium experimental murine colitis,» Laboratory Investigation, vol. 69, no. 2, pp. 238 - 249, 1993.

Full text is published :
Kim A.D., Lepekhova S.A., Chashkova Ye.Yu., Gol’dberg O.A., Shedoeva L.R. TECHNOLOGY SURGICAL INTERVENTION IN ULCERATIVE COLITIS MODELING DESCENDING PART OF THE COLON IN RATS LINE «WISTAR». Experimental and Clinical Gastroenterology Journal. 2016;129(05):28-32
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