1. KSMA - Branch Campus of the FSBEI FPE RMACPE MOH Russia (Kazan, Russian Federation)
2. REGIONAL clinical hospital № 7 (Kazan, Russian Federation)

Keywords: acute pancreatitis,protease,protease activity,sera,urine,zymography method

Abstract:The aim of the investigation was to study the spectrum of proteolytic enzymes and their distribution by molecular mass and type of active centers in the urine and serum of patients with acute pancreatitis. Materials and methods. The study included 48 patients with acute pancreatitis and 19 healthy volunteers. In patients and in healthy was determined presence and activity of proteolytic enzymes in urine and serum gelatin gel zymography method. Results. In the urine of patients with acute pancreatitis, no enzymes with proteolytic activity were found. All healthy volunteers we showed the presence of three protease with molecular masses of 170, 144 and 121 kDa, patients had an additional protease - 121 kDa (n=24), 53 kDa, 42 kDa (n=5) and 23-26 kDa (n=18) in serum. The protease 121 kDa was presented in 50 % of patients of mild, moderate and severe degrees of severity. Protease 53kDa, 42kDa and 23-26 kDa were more often in severe patients. Protease activity of 170 kDa and 112 kDa was significantly higher (p <0.05) than in healthy volunteers and decreased during the treatment. Protease that were absent in healthy volunteers (121, 23и 26 kDa) also decreased its activity in the treatment process, but the convalescence of patient was not accompanied by the disappearance of their activity. Conclusions. In sera of patients with acute pancreatitis, a broader spectrum of proteases was found in comparison with healthy volunteers. In the urine of patients with acute pancreatitis, no enzymes with proteolytic activity were found.

• References total 8 ⇓ :: .
  1. Hirota M., Ohmuraya M., and H. Baba. /Genetic background of pancreatitis.// Postgrad. J. Med, 2006 82: 775-778
  2. Johnson C. D., Lempinen M., Imrie C. W., P. Puolakkainen, E. Kemppainen, R Carter. and C. McKay. / Urinary trypsinogen activation peptide as a marker of severe acute pancreatitis // British Journal of Surgery 2004; 91: 1027-1033
  3. Geert W. Schmid-Schönbein. / The autodigestion hypothesis: Proteolytic receptor cleavage in rheological and cardiovascular cell dysfunction1// Biorheology -1 (2017) 1-13
  4. Chen P., Yuan Y., Wang S., Zhan L., Xu J. / Serum matrix metalloproteinase 9 as a marker for the assessment of severe acute pancreatitis // Tohoku J Exp Med. 2006 Mar;208(3):261-6
  5. Guo J.1, Xue P., Yang X.N, Liu X.B, Huang W., Xia Q. / Serum matrix metalloproteinase-9 is an early marker of pancreatic necrosis in patients with severe acute pancretis // Hepatogastroenterology. 2012 Jul-Aug;59(117):1594-8
  6. Johnson C. D., Lempinen M., Imrie C. W., P. Puolakkainen, E. Kemppainen, R Carter. and C. McKay. / Urinary trypsinogen activation peptide as a marker of severe acute pancreatitis // British Journal of Surgery 2004; 91: 1027-1033
  7. Elin Hadler-Olsen, Bodil Fadnes, Ingebrigt Sylte, Lars Uhlin-Hansen, Jan-Olof Winberg. / Regulation of matrix metalloproteinase activity in health and disease // FEBS Journal Volume 278, Issue 1, pages 28-45, January 2011
  8. Bu C. H., Pourmotabbed T / Mechanism of Ca2+ dependent activity of human neutrophil gelatinase // J BiolChem 271-14308-14315. 1996

Full text is published :
Zinkevich O.D., Safina N.A., Chikaev V.F., Malkov T.S. et al. PROTEOLYTIC ENZYMES IN URINE AND BLOOD SERUM IN PATIENTS WITH ACUTE PANCREATITIS. Experimental and Clinical Gastroenterology Journal. 2017;147(11):86-91
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