1. Institute of Biology, Chemistry and Bioresources of Chernovtsi national university named by Yurii Fedkovych, Biochemistry and biotechnology department (Chernivtsi, Ukraine)

Keywords:acetaminophen-induced hepatitis,alimentary deprivation of protein,indicators of hemostasis system

Abstract:The hemostasis system indicators of rats with acetaminophen-induced hepatitis under the conditions of alimentary deprivation of protein were assessed in the research. Research was conducted on 4 groups of animals: 1 - control; 2 - rats maintained on low-protein diet; 3 - rats with acute acetaminophen-induced hepatitis, maintained on full-value ration, 4 - rats with acute acetaminophen-induced hepatitis, maintained under the conditions of alimentary deprivation of protein. The activated partial thromboplastin time (APTT), prothrombin time (PT), thrombin time (TT) and fibrinogen concentration were determined by optical method on a semi-automated coagulometer HUMACLOT Junior (Human GmbH, Germany). The content of soluble fibrin-monomer complexes (SFMC) was assessed using the “RFMK test” test system; the serum D-dimer concentration was determined by enzyme immunoassay. Our study established an increase of prothrombin time in protein-deficient animals, while in other parameters of the hemostasis system significant changes were not detected. Meanwhile, a significant increase of APTT, prothrombin and thrombin time is established in animals with acetaminophen-induced hepatitis. At the same time, the results of this study suggest that the accumulation of blood fibrinogen and SFMC is higher in almost 3 and 1.5 times respectively compared to control values, on the background of maintaining of the D-dimer concentration at the control level. The most pronounced changes of hemostatic system are observed in animals with toxic liver damage, which contained on a low protein diet. A decrease of procoagulant blood potential was established in rats of this group that was shown by increase of APTT, prothrombin time and also of hyperfibrinogenemia on the background of the accumulation of SFMC. In conclusion, acetaminophen-induced liver damage is accompanied by disorders of the hemostasis system, which are most pronounced in protein-deficient animals. The established elongation of APTT, PT, TT and the increasing of fibrinogen and SFMC contents indicate the formation of the hypocoagulation state and risk of hemorrhage. The results of the research can be used for biochemically substantiate and the development of optimal strategy for the correction of coagulopathies as consequences of toxic liver damage..

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Full text is published :
THE STATE OF HEMOSTASIS SYSTEM IN RATS WITH TOXIC HEPATITIS UNDER THE CONDITIONS OF ALIMENTARY DEPRIVATION OF PROTEIN. Experimental and Clinical Gastroenterology Journal. 2018;150(02):82-88
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