1. FSBU “National Almazov Medical Research Centre”, Ministry of Health, Russia (Saint Petersburg, Russia)

Keywords: antibiotic-induced intestinal dysbiosis, isolated heart, ischemia, infarct size

Abstract: Aim: assessment of morphological and functional parameters of the isolated heart subjected to global ischemia-reperfusion in conventional and SPF rats with antibiotic-induced intestinal dysbiosis (AID). Materials and methods: experiments were performed on male Wistar rats belonging to conventional and SPF colonies. AID was induced by intragastric administration of clarithromycin and metronidazole during 3 days. Results: SPF animals demonstrated more severe disorders in the evacuatory function of the gastrointestinal tract. In addition, important diff erences in baseline myocardial function and myocardial tolerance to ischemia-reperfusion were noted between SPF and conventional rats. Conclusion: conventional animals are characterized by increased myocardial tolerance to ischemia-reperfusion injury versus SPF animals, which might be attributed to the presence of chronic low-grade systemic infl ammatory response causing activation of cardioprotective signaling in conventional rodents.

• References total 13 ⇓ :: .
  1. Postler T.S., Ghosh S. Understanding the holobiont: how microbial metabolites aff ect human health and shape the immune system // Cell. Metab. – 2017. – Vol. 26. – P. 110–130.
  2. Raymond F., Ouameur A. A., Deraspe M. et al. Th e initial state of the human gut microbiome determines its reshaping by antibiotics // ISME J. – 2016. – Vol. 10. – P. 707–720.
  3. De Vos W. M., de Vos E. A. Role of the intestinal microbiome in health and disease: from correlation to causation // Nutr. Rev. – 2012. – Vol. 70. – P. S45-S56.
  4. Averina O. V., Ermolenko E. I., Ratushniy A. Y., Tarasova E. A., Borschev Y. Y., Leontieva G. F., Kramskaya T. A., Kotyleva M. P., Danilenko V. N., Suvorov A. N. Infl uence of probiotics on cytokine production in the in vitro and in vivo systems. Medical Immunology (Russia). 2015;17(5):443– 454. https://doi.org/10.15789/1563–0625–2015–5–443–454
  5. Lazebnik L. B., Sokolova G. N., Cherniaev A. Ia. Chronic ulcers in elderly people. Eksp Klin Gastroenterol. 2002;(1):3–7.
  6. Borshcev Y. Y., Minasian S. M., Burovenko I. Y., Ermolenko E. I., et al. Infl uence of probiotic strain e. Faecium l3 on myocardial tolerance to ischemia-reperfusion injury in the model of antibiotic-induced intestinal dysbiosis. Ross Fiziol Zh Im I M Sechenova. 2016 Nov;102(11):1323–32.
  7. Korolev D. V., Aleksandrov I. V., Galagudza M. M. et al. Automation of the acquisition and processing of physiological experiment data. Regional blood circulation and microcirculation. 2008, vol. 7, no 2(26), pp. 79–84
  8. Minasyan S. M., Badrikhanova L. R., Galagudza M. M. et al. Comparative study of the protective eff ect of hypothermia, ischemic preconditioning and modifi ed cardioplegic solutions in ischemia-reperfusion of an isolated rat heart. Regional blood circulation and microcirculation. 2008, vol. 7, no 2(26), pp. 72–78
  9. Wlodarska M., Willing B., Keeney K. M. et al. Antibiotic treatment alters the colonic mucus layer and predisposes the host to exacerbated Citrobacter rodentium-induced colitis // Infection and immunity. – 2011. – Vol. 79. – P. 1536–1545.
  10. Borshcev Y. Y., Gromova L. V., Ermolenko E. I., et al. Reaction of small and large intestine peptidhydrolases of rats to antibiotic administration. Ross Fiziol Zh Im I M Sechenova. 2016 Nov;102(11):1323–32.
  11. Hoshida S., Yamashita N., Otsu K., Hori M. Repeated physiologic stresses provide persistent cardioprotection against ischemia-reperfusion injury in rats // J. Am. Coll. Cardiol. – 2002. – Vol. 40. – P. 826–831.
  12. Hoshida S., Yamashita N., Otsu K., Hori M. Th e importance of manganese superoxide dismutase in delayed preconditioning: involvement of reactive oxygen species and cytokines // Cardiovasc. Res. – 2002. – Vol. 55. – P. 495–505.
  13. Lam V., Su J., Koprowski S., Hsu A. et al. Intestinal microbiota determine severity of myocardial infarction in rats // FASEB J. – 2012. – Vol. 26. – P. 1727–1735.

Full text is published :
Borshchev Yu. Yu., Minasian S. M., Burovenko I. Yu., Borshchev V. Yu., Galagudza M. M. Antibiotic–induced intestinal dysbiosis and myocardial tolerance to ischemia-reperfusion injury in rats with diff erent microbiological status. Experimental and Clinical Gastroenterology. 2018;158(10): 62–67. DOI: 10.31146/1682-8658-ecg-158-10-62-67
Read & Download full text