1. State Scientifi c Center of Coloproctology, 123423, Moscow, Russia, st. Salam Adil, 2

Keywords: Clostridium (Clostridioides) diffi cile, antioxidant activity, reactive oxygen species, chemiluminescence

Abstract:Aim: to develop and to evaluate a method for Clostridium (Clostridioides) diffi cile antioxidant activity (AOA) assessment via chemiluminescent (CL) analysis. Materials and methods: we investigated ten C. diffi cile strains, obtained from patients with antibiotic-associated diarrhea that were cultured for two days. AOA was assessed on the fi rst and second day of cultivation by means of CL assay based on 2,2’-azo-bis (2-amidinopropane) dihydrochloride-luminol system. Results: our method for AOA estimation is characterized by high reproducibility and reliability (the average coeffi cient of variation between parallel samples is 2.0–18.2%, the calibration curve coeffi cient of determination is 80.5–89.4%). C. diffi cile AOA after the fi rst day of cultivation is 6.95–9.93 units; it increases to 9.86–13.27 units after the second day (p <0.01). A negative correlation (η = –0.3, p <0.05) is shown between the initial AOA level and its growth rate during cultivation. Conclusion: we propose a simple and reliable method for C. diffi cile AOA assessment that is suitable for routine use in clinical bacteriology. Length of C. diffi cile culturing is associated with increase of its AOA.

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Full text is published :
Obraztsov I. V., Sukhina M. A. Evaluation of antioxidant activity of Clostridium (Clostridioides) diffi cile. Experimental and Clinical Gastroenterology. 2018;160(12): 64–69. (In Russ.) DOI: 10.31146/1682-8658-ecg-160-12-64-69
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